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fitc conjugated anti mucin 16  (Assaypro)


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  • 90

    Structured Review

    Assaypro fitc conjugated anti mucin 16
    Fitc Conjugated Anti Mucin 16, supplied by Assaypro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+conjugated+anti+mucin+16/Human+Mucin-16+(MUC-16%2C+CA-125)+AssayLite+Antibody/pmc07876453-142-23-25
    Average 90 stars, based on 1 article reviews
    fitc conjugated anti mucin 16 - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Targeting Indoleamine 2,3-Dioxygenase in Cancer Models Using the Novel Small Molecule Inhibitor NTRC 3883-0
    Article Snippet: The cell samples were then cultured for 2–3 weeks in advanced RPMI medium (ThermoFisher), supplemented with 10% FBS, 1% glutamax and 1% P/S, with 2 or 3 times passaging of the cells. qPCR analyses of the MUC16, HE4, IDO1, and TDO2 genes were performed for all samples as described above. .. Five samples which could be cultured over multiple passages were analyzed for cell surface expression of mucin-16 and EpCAM by flow cytometry using FITC-conjugated anti-mucin-16 (AssayPro, #32189-05141) and APC-conjugated anti-EpCAM (Miltenyi, #130-111-117, RRID: AB_2657496) to assure that the cell cultures consisted of malignant cells. .. IDO1 mRNA and protein levels in these samples were analyzed as described above after incubation with or without 200 ng/ml IFNγ for 24 h. The collection of ascites and the research described was conducted with approval of the medical ethical committee of the Radboud university medical center and informed written consent from each subject.

    Expressing:

    Article Title: Targeting Indoleamine 2,3-Dioxygenase in Cancer Models Using the Novel Small Molecule Inhibitor NTRC 3883-0
    Article Snippet: The cell samples were then cultured for 2–3 weeks in advanced RPMI medium (ThermoFisher), supplemented with 10% FBS, 1% glutamax and 1% P/S, with 2 or 3 times passaging of the cells. qPCR analyses of the MUC16, HE4, IDO1, and TDO2 genes were performed for all samples as described above. .. Five samples which could be cultured over multiple passages were analyzed for cell surface expression of mucin-16 and EpCAM by flow cytometry using FITC-conjugated anti-mucin-16 (AssayPro, #32189-05141) and APC-conjugated anti-EpCAM (Miltenyi, #130-111-117, RRID: AB_2657496) to assure that the cell cultures consisted of malignant cells. .. IDO1 mRNA and protein levels in these samples were analyzed as described above after incubation with or without 200 ng/ml IFNγ for 24 h. The collection of ascites and the research described was conducted with approval of the medical ethical committee of the Radboud university medical center and informed written consent from each subject.

    Flow Cytometry:

    Article Title: Targeting Indoleamine 2,3-Dioxygenase in Cancer Models Using the Novel Small Molecule Inhibitor NTRC 3883-0
    Article Snippet: The cell samples were then cultured for 2–3 weeks in advanced RPMI medium (ThermoFisher), supplemented with 10% FBS, 1% glutamax and 1% P/S, with 2 or 3 times passaging of the cells. qPCR analyses of the MUC16, HE4, IDO1, and TDO2 genes were performed for all samples as described above. .. Five samples which could be cultured over multiple passages were analyzed for cell surface expression of mucin-16 and EpCAM by flow cytometry using FITC-conjugated anti-mucin-16 (AssayPro, #32189-05141) and APC-conjugated anti-EpCAM (Miltenyi, #130-111-117, RRID: AB_2657496) to assure that the cell cultures consisted of malignant cells. .. IDO1 mRNA and protein levels in these samples were analyzed as described above after incubation with or without 200 ng/ml IFNγ for 24 h. The collection of ascites and the research described was conducted with approval of the medical ethical committee of the Radboud university medical center and informed written consent from each subject.



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    90
    Assaypro fitc conjugated anti mucin 16
    Fitc Conjugated Anti Mucin 16, supplied by Assaypro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+conjugated+anti+mucin+16/Human+Mucin-16+(MUC-16%2C+CA-125)+AssayLite+Antibody/pmc07876453-142-23-25
    Average 90 stars, based on 1 article reviews
    fitc conjugated anti mucin 16 - by Bioz Stars, 2026-10
    90/100 stars
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